ABSTRACT
Objective:To establish a method that could determine 7 chemical components including baicalin, berberine hydrochloride, aloe-emodin, rhein, emodin, chrysophanol and physcion in San huang tablet by high performance liquid chromatography-diode array detector (HPLC-DAD) method simultaneously with quantitative analysis method. Methods:The Diamonsil C18 column (4.6 mm × 250 mm, 5 μm) was adopted. The mobile phase was acetonitrile (A)-0.1% phosphoric acid solution (B) with gradient elution at a flow rate of 1.0 ml/min. The column temperature was set at 30 ℃. The detector was DAD. The detection wavelengths were 280 nm (baicalin), 265 nm (berberine hydrochloride), 254 nm (aloe-emodin, rhein, emodin, chrysophanol and physcion).Results:The linear ranges of baicalin, berberine hydrochloride, aloe-emodin, rhein, emodin, chrysophanol and physcion were within the ranges of 0.051 8-0.518 0 μg ( r=0.999 2), 0.024 6-0.245 9 μg ( r=0.999 8), 0.002 0- 0.020 2 μg ( r=0.999 6), 0.002 0-0.020 0 μg ( r=0.999 0), 0.002 0-0.020 1 μg ( r=0.999 9), 0.002 0-0.020 1 μg ( r=0.999 5), and 0.001 0-0.010 2 μg ( r=0.999 8), respectively. The average recoveries ( n=6) were 95.41%-100.59%, with RSDs less than 3.11%. Conclusions:This HPLC-DAD method is consistent with methodology and could simultaneously determine 7 chemical components in San huang tablet.